GdmCl- and Urea-induced Unfolding Parameters for the hFXN Variants.

<p>Parameter m_NU is the slope of the linear dependency of free energy of unfolding (ΔG_NU°) on denaturant concentration and ΔG°_{NU H2O} is ΔG°_NU at zero denaturant concentration. In the fittings, we used the predicted m_NU values for GdmCl- and urea-induced unfolding considering a globular protein of a given molecular weigtht, 2.9 and 1.45 kcal mol⁻¹ M⁻¹ for hFXN90–210 and 2.5 and 1.25 kcal mol⁻¹ M⁻¹ for hFXN90–195 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045743#pone.0045743-Myers1" target="_blank">[68]</a>. The units of m_NU are kcal mol⁻¹ M⁻¹. C_m and free energy are M and kcal mol⁻¹, respectively. CD signals are in [Θ] ×1 10⁻³ (deg cm² dmol⁻¹), and fluorescence intensity signals are arbitry units. The parameters were calculated by nonlinear least square fit of the data shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045743#pone-0045743-g004" target="_blank">Figure 4</a> as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045743#s4" target="_blank">Materials and Methods</a>.