<i>In vivo</i> validation of the pluripotency of 1LMF-h-iPS 1 and 1LMF-h-iPS 2 cells.

<p>Teratoma formation in mice and tissue sections showing the formation of cells from the three germ layers: endoderm (gut epithelium and secretory epithelium), mesoderm (adipose, bone, cartilage, and striated muscle fibers), and ectoderm (epidermis and neural rosettes) in 1LMF-h-iPS 1 (A) and 1LMF-h-iPS 2 cells (B). (C and C’) Chimeric mouse produced by injecting 1LMF-h-iPS 2 cells into c57BL/6 host blastocysts. The natural coat color of the surrogate strain ICR is white, and the brown hair (red arrowhead) indicates a chimeric mouse (red) that originated from the 1LMF-h-iPS 2 cells.