<i>Creface^T/T</i> embryos exhibit brain anomalies.

<p><i>Pax6</i> whole embryo <i>in situ</i> hybridization in E9.5 control (A, C, E, G) and <i>Creface^T/T</i> (B, D, F, H) embryos demonstrating ventrally displaced optic cup (arrows, arrowheads) and agenesis of prosomere 2 in the diencephalon (parentheses) of mutant embryos. Hematoxylin and eosin stained transverse sections through the forebrain and eye regions of control (I, K) and <i>Creface^T/T</i> (J, L) embryos reveal absence of the ventricle due to bifurcation failure (asterisk in I, J) and agenesis of the cornea in association with microphthalmia (asterisk in L) in mutants. Abbreviations: C, cornea; DI, diencephalon; LE, lens; LGE, lateral ganglionic eminence; MGE, medial ganglionic eminence; OP, optic placode; P, prosomere; RE, retina; TE, telencephalon. Scale bars: A–H 500 uM; I–L 200 uM.