E111 in complex with DENV-1 DIII.

<p>A ribbon diagram of the crystal structure of (<b>A</b>) E111 scFv in complex with DENV-1 strain 16007 DIII and (<b>B</b>) E111 Fab in complex with DENV-1 strain West Pac-74 DIII. The light chain is colored in cyan and the heavy chain in magenta. 16007 DIII is colored in light gray and West Pac-74 DIII is in gray. (<b>C</b>) A surface model showing the contact residues in the scFv complex. Contacts in the scFv are highlighted by light chain (cyan) or heavy chain (magenta) contacts. DIII contacts are highlighted by heavy chain (magenta), light chain (cyan), or both chains (green). (<b>D</b>) A ribbon diagram showing the residues of DIII contacted by the E111 scFv in the crystal structure. DIII residues that are contacted by the heavy and light chains of E111 (338, 344, and 345) are labeled. (<b>E</b>) A close-up of the contacts made by E111 in the CC′ loop of 16007 DIII (yellow) or West Pac-74 DIII (white). (<b>F</b>) Sequence of the four segments of 16007 DIII contacted by E111 aligned with the analogous residues of the other four DENV-1 genotypes and three serotypes. Residues are colored as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002930#ppat-1002930-g001" target="_blank"><b>Figure 1D</b></a>.