Genetic structure of MES_PM1 carrying four resistance genes, <i>ermB</i>, <i>aph(3′)-IIIa</i>, <i>aadE</i>, and <i>cat</i>.

<p>MES_PM1 was integrated into and disrupted the <i>sasK</i> gene, which encoded a putative cell-wall anchored surface protein with the LPXTG-motif. The intact <i>sasK</i> gene was present in PVL-positive ST59 CA-MRSA type strain USA1000. Three <i>att</i> sites are in a vertical red line. The region of IS<i>1216V</i> with the transposase gene (<i>tnp</i>) is shaded in blue. Homology regions with <i>E. faecalis</i> pLG2 plasmid (GenBank accession number HQ426665) and <i>S. aureus</i> SAP084A (GenBank accession number GQ900436) are shaded in yellow and purple, respectively. A Greek delta symbol indicates truncated coding sequence.