Low doses of γIR, CQ and PI-103 synergistically induce apoptosis in GBM22 SLGCs.

<p>(<b>A, B</b>) Left: Percentage of annexin V/PI positive cells 5 or 13 d after treatment with either γIR (3.5 Gy), CQ (5 µM) or PI-103 (0.5 µM) alone, with double or triple combinations. Right: cell numbers after trypan blue staining. Lower panel in A: flow cytometry data showing that cell death occurred primarily through apoptosis. (<b>C</b>) Western blot analysis of LC3-I/II conversion and of p21 expression levels.