YTS-CD2-GFP cells can simultaneously form multiple NTs.

<p>YTS-CD2-GFP cells were incubated with 721 cells for 30 minutes on anti-CD48 coated plates then imaged live using a spinning disc confocal microscope at a single time point. (A) Multiple NTs form between a single NK and target cell, or (B) connect an NK cell to multiple target cells. (C) The number of NK cells forming multiple NTs is inversely proportional to the number of NTs per cell (n>100 NTs). (D) As the number of NTs per cell increases, the average NT length decreases (1–3 NTs/Cell: n>25 NTs; 4 NTs/Cell: n>5 NTs). (Scale bars: 5 µm.)