CD2 receptor-ligand interactions promote NT formation in eNK cells.

<p>(A) NTs were visualized in PKH26 labeled eNK cells isolated from whole blood. (B) eNK cells were labeled with PKH26, treated with isotype or anti-CD2 blocking antibody for 15 minutes, then incubated with 721 cells on anti-CD48 coated plates for 30 minutes. Single images were obtained to determine NT frequency (n>850 cells). (C) eNK cells were sorted into CD2⁺ and CD2⁻ populations using a non-blocking antibody to CD2. (D) CD2⁺ and CD2⁻ eNK populations were labeled with PKH26, and single images were obtained to determine the frequency of NK-NK (black bar) and NK-TC (grey bar) NT formation (n>325 cells). (Scale bar: 5 µm.).