KLF4 enhances the DNA-binding affinity of p53.

<p>A: DNA constructs generated and principle of cooperative fluorescence anisotropy titrations. Fluorescein (*, star), a p53 RE (<i>P</i>, dark grey), a spacer (<i>n</i>, light grey), and a KLF4 RE (<i>K</i>, black) compose the labelled DNA. The affinity of p53 towards DNA is measured in the presence (+ KLF4) and absence (- KLF4) of KLF4. B: Example p53 titration data using *P30K as DNA in the presence (circles) and absence (squares) of KLF4 in FA285 buffer. C: Normalised p53 DNA-binding affinity in the presence of 400 nM KLF4 as a function of the distance between the p53 RE and the KLF4 RE.