Nuclear localization of FOXA2 is independent of sumoylation.

<p>INS-1E cells plated on cover slips were not transfected (panels L,M,N) or transfected with pCGN empty vector (panels A,B,C) or HA epitope tagged FOXA2 (panels D,E,F,G) or FOXA2K6R mutant (panels H,I,J,K,O). Cells were fixed 36 hours post-transfection, permeabilized and stained with mouse HA antibody and fluorescent Alexa Fluor 594 donkey anti-mouse secondary antibody. No primary antibody was used for panels L,M,N. Cover slips were mounted in DAPI containing mounting media. Panels B,E,I and M are DAPI images. Panel C is superimposed A and B images. Panel F is superimposed D and E images. Panel J is superimposed H and I images. Panel N is superimposed L and M images. All images are at 400× magnification. Cells in the marked boxes in images F and J are digitally magnified and shown in panels G,K and O. Four times longer exposure were used for panels A, H and L. Two times lesser exposure was used for panel I.