Differential expansion of Th1 and Th17 populations after adoptive transfer.

<p>Th1 or Th17 recipient mice were immunised with HEL-OVA in CFA or PBS and the expansion of transgenic T cells and their ability to produce IL-17 and IFNγ was analysed by FACS. Representative FACS plots demonstrating gating strategy are demonstrated (A). Collective data demonstrating the percentage (B) and number of (C) of transgenic T cells at days 3, 7 and 10 post-immunisation amongst draining lymph node cells was assessed by flow cytometry, based on the expression of CD4 and the clonotypic TcR recognised by the KJ1.26 antibody (A). Unimmunised controls from each time point were averaged and represented as day 0. At the same days cells from the dLNs were stimulated with PMA and ionomycin and the expression of IL-17 (D) and IFNγ (E) by the transferred transgenic T cells was assessed by flow cytometry. Day 0 represents the proportion of IL-17 or IFNγ population in the transferred polarised populations. Grey line represents Th17 immunised recipients, grey dotted line unimmunised Th17 recipients, black line Th1 immunised recipients and black dotted line Th1 unimmunised recipients. Data represent mean ±SEM. *: Th1/HEL-OVA vs. Th17/HEL-OVA, *p<0.05, **p<0.01, ***p<0.001 (n = 3). Similar results were obtained in two additional experiments.