<p>Control (Ctrl) and IL-4 (10 ng/ml), IL-13 (10 ng/ml), IFNγ (50 ng/ml), LPS (100 ng/ml), and 3M-002 (3 µM) stimulated macrophages were allowed to migrate towards the chemotactic factor fLMF (+). Medium without chemotactic factor (−) was used as control for basal migration in each experiment. Graphs show the mean migration index compared to each individual control (n = 3) and error bars display the standard deviation (±SD). *p<0.05; **p<0.01.</p
While many tools exist to study immune-cell chemotaxis in vitro, current methods often lack desirabl...
<p>To test that the assay was detecting true macrophage chemotaxis, rather than chemokinesis or fuge...
<p>(A) Transwell assay showed that conditioned-medium from macrophages treated with Wnt5a expression...
<p>Bio-Gel elicited macrophages from C57BL/6J (4×10<sup>5</sup> total) were allowed to migrate towar...
<p>To determine the chemotactic potential of macrophages for MSCs the CM from primary culture macrop...
<div><p>Chemotaxis assays are an invaluable tool for studying the biological activity of inflammator...
<p>Macrophage migration assays using DMEM (migration media) or conditioned medium (CM) collected fro...
Chemotaxis assays are an invaluable tool for studying the biological activity of inflammatory mediat...
<p>Panels <b>A, D, G & J</b> are representative FACS plots of the four different murine macrophage p...
The cloned mouse macrophage cell line, BAC1.25F, resembles primary macrophages in its dependence on ...
<p>Thioglycollate and Bio-Gel elicited macrophages (4×10<sup>5</sup> total) were allowed to migrate ...
(A) Transwell migration of THP-1 macrophage in response to the cytokine secretion of ARPE-19 cells e...
<p>Probenecid reduces the media-induced recruitment of macrophage. (a) Schematic depiction of the ch...
<p>Migration of PBMCs (A) in response to supernatants from uninfected macrophages (black), macrophag...
<p>PBMC migration was evaluated in 96 well Transwell-plates (pore size: 5 μm). Medium containing 10%...
While many tools exist to study immune-cell chemotaxis in vitro, current methods often lack desirabl...
<p>To test that the assay was detecting true macrophage chemotaxis, rather than chemokinesis or fuge...
<p>(A) Transwell assay showed that conditioned-medium from macrophages treated with Wnt5a expression...
<p>Bio-Gel elicited macrophages from C57BL/6J (4×10<sup>5</sup> total) were allowed to migrate towar...
<p>To determine the chemotactic potential of macrophages for MSCs the CM from primary culture macrop...
<div><p>Chemotaxis assays are an invaluable tool for studying the biological activity of inflammator...
<p>Macrophage migration assays using DMEM (migration media) or conditioned medium (CM) collected fro...
Chemotaxis assays are an invaluable tool for studying the biological activity of inflammatory mediat...
<p>Panels <b>A, D, G & J</b> are representative FACS plots of the four different murine macrophage p...
The cloned mouse macrophage cell line, BAC1.25F, resembles primary macrophages in its dependence on ...
<p>Thioglycollate and Bio-Gel elicited macrophages (4×10<sup>5</sup> total) were allowed to migrate ...
(A) Transwell migration of THP-1 macrophage in response to the cytokine secretion of ARPE-19 cells e...
<p>Probenecid reduces the media-induced recruitment of macrophage. (a) Schematic depiction of the ch...
<p>Migration of PBMCs (A) in response to supernatants from uninfected macrophages (black), macrophag...
<p>PBMC migration was evaluated in 96 well Transwell-plates (pore size: 5 μm). Medium containing 10%...
While many tools exist to study immune-cell chemotaxis in vitro, current methods often lack desirabl...
<p>To test that the assay was detecting true macrophage chemotaxis, rather than chemokinesis or fuge...
<p>(A) Transwell assay showed that conditioned-medium from macrophages treated with Wnt5a expression...