Microarray analysis of gene expression changes in Müller cells post-ESMV treatment.

<p>(A) Venn diagram of gene expression changes in ESMV-treated vs. control Müller cells at 8, 24, and 48 hours post ESMV exposure. The expression of 1894 genes was altered by ESMV treatment at all three time-points. There was significant overlap in differentially regulated genes between 8 and 24 hours (192) and 24 and 48 hours (853) post-treatment. The majority of gene expression changes occurred by 24 hours post-ESMV exposure. (B) Heat map representation of heirarchal clustering of 16 samples based on the 1894 probes found to be differentially regulated in the Müller cells post-ESMV treatment vs. control (p-value<0.001 and a minimum of 3-fold difference in expression). These transcripts were clustered with conventional Heat map analysis, with red representing up- and blue representing down-regulation; rows represent the samples and columns represent the genes.