Competitive inhibition of PvTRAgs binding to erythrocytes by

<p><b>Cell ELISA.</b> A mixture of Histidine-tagged recombinant PvTRAg (200 nM) and variable amount (0.0 to 2 µM) of respective untagged PvTRAg was added to ∼1 million RBCs. After incubation, the plates were developed by the anti-histidine antibodies as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050754#pone-0050754-g001" target="_blank">Fig.1</a> legend. a, PvTRAg35.2; b, PvTRAg69.4; c, PvTRAg38; d, PvTRAg33.5; e, PvATRAg74; f, PvTRAg. Data shown are the mean ± S.D. of at least 3 independent experiments.