Cell-ELISA based cross-inhibition of PvTRAgs binding to erythrocytes by rabbit antibodies raised against the same antigen or against its close homolog.

*<p>In a cell-ELISA assay, the Histidine tagged PvTRAgs were incubated with polyclonal antisera raised in rabbits at 1∶100 dilutions against the same antigen or its closest homolog (shown in brackets). Pre-incubated mixture was then allowed to react with erythrocytes and binding was detected by monoclonal anti-His₆ antibody as described in the text. Binding of PvTRAgs in the absence of antibody is taken as100%. Values are mean ± standard deviation of three different experiments. The erythrocyte binding inhibition between self antibody and homologue antibody was significantly different (P<0.05).