The effect of soluble glycans on T1L infectivity of MEFs.

<p>(<b>A,B</b>) T1L or (<b>C</b>) T3D (10⁷ PFU/well) were pre-incubated with the GM2 (<b>A,C</b>) or GM3 (<b>B</b>) glycan at the concentrations shown for 1 h prior to adsorption to MEFs at a final MOI of 100 PFU/cell. Cells were washed twice with PBS, and fresh medium was added. After incubation at 37°C for 20 h, cells were fixed and reovirus antigen was detected by indirect immunofluorescence. Nuclei were quantified by DAPI staining. The results are expressed as the mean percent infected cells per field in triplicate wells for two independent experiments. Error bars represent standard deviations. *, <i>P</i><0.05; **, <i>P</i><0.01; ***, <i>P</i><0.0001, as determined by two-tailed Student's <i>t</i> test.