<p>Monolayers of MEFs were adsorbed with the strains shown at an MOI of 1 FFU/field (as titered in L cells) at room temperature for 1 h. Cells were washed twice with PBS, and fresh medium was added. After incubation at 37°C for 20 h, cells were fixed, and reovirus antigen was detected by indirect immunofluorescence. Nuclei were stained with DAPI. The percentage of infected cells in three fields of view per well was determined. The results are from a representative experiment of three experiments performed with triplicate wells. Error bars represent standard deviations. **, <i>P</i><0.01, as determined by two-tailed Student's <i>t</i> test.</p
(A) At 96 h post-transfection with siRPAP3, cell viability was determined and calculated as a percen...
<p>(A) Viability assay (WST-1) on 911 and U118MG cells. Cells were mock infected (black bar) or infe...
At 48 h post-transfection with either siRPAP3 or siNC, A549/SeV-C and A549/Ctrl cells were infected ...
<p>(A) L929 cells were adsorbed with rsT3D or the indicated φ mutant at an MOI of 10<sup>4</sup> par...
<p>(<b>A</b>) Top-view of T1L σ1 (gray) in complex with the GM2 glycan (yellow). Residues Q417 and G...
<p>(A) Several cell lines were infected with <i>wt</i> T3D or <i>jin-1</i> and 32 hr post-infection ...
<p>(<b>A,B</b>) T1L or (<b>C</b>) T3D (10<sup>7</sup> PFU/well) were pre-incubated with the GM2 (<b>...
<p>(A) VIMC, CoMS, CM-MC, HRMC, RBL-2H3 and P815 cells in triplicate wells were either mock-infected...
<p>(<b>A</b>) L cells or (<b>B</b>) MEFs were treated with <i>A. ureafaciens</i> neuraminidase for 1...
<p>Purified reovirus virions (10<sup>11</sup> particles) were serially diluted in 0.05 ml of PBS in ...
<p>Each of five different cell lines (L929, A549, HEK293, CaCo<sub>2</sub> and Hela) were infected a...
<p>(A, B) Reovirus T1L and T3D were not incubated, incubated with PBS, detoxified LPS (dLPS), LPS, o...
<p>(A) CMP-sialic acid transporter defective Lec2 cells and parental cell line, CHO, were exposed to...
<p>Reovirus T1L virions (5×10<sup>3</sup> particles) or ISVPs (1×10<sup>3</sup> particles) was not i...
<p>(A) Cell viability of reovirus-infected (MOI 70) canine MCT cell lines were assessed with 0.25% t...
(A) At 96 h post-transfection with siRPAP3, cell viability was determined and calculated as a percen...
<p>(A) Viability assay (WST-1) on 911 and U118MG cells. Cells were mock infected (black bar) or infe...
At 48 h post-transfection with either siRPAP3 or siNC, A549/SeV-C and A549/Ctrl cells were infected ...
<p>(A) L929 cells were adsorbed with rsT3D or the indicated φ mutant at an MOI of 10<sup>4</sup> par...
<p>(<b>A</b>) Top-view of T1L σ1 (gray) in complex with the GM2 glycan (yellow). Residues Q417 and G...
<p>(A) Several cell lines were infected with <i>wt</i> T3D or <i>jin-1</i> and 32 hr post-infection ...
<p>(<b>A,B</b>) T1L or (<b>C</b>) T3D (10<sup>7</sup> PFU/well) were pre-incubated with the GM2 (<b>...
<p>(A) VIMC, CoMS, CM-MC, HRMC, RBL-2H3 and P815 cells in triplicate wells were either mock-infected...
<p>(<b>A</b>) L cells or (<b>B</b>) MEFs were treated with <i>A. ureafaciens</i> neuraminidase for 1...
<p>Purified reovirus virions (10<sup>11</sup> particles) were serially diluted in 0.05 ml of PBS in ...
<p>Each of five different cell lines (L929, A549, HEK293, CaCo<sub>2</sub> and Hela) were infected a...
<p>(A, B) Reovirus T1L and T3D were not incubated, incubated with PBS, detoxified LPS (dLPS), LPS, o...
<p>(A) CMP-sialic acid transporter defective Lec2 cells and parental cell line, CHO, were exposed to...
<p>Reovirus T1L virions (5×10<sup>3</sup> particles) or ISVPs (1×10<sup>3</sup> particles) was not i...
<p>(A) Cell viability of reovirus-infected (MOI 70) canine MCT cell lines were assessed with 0.25% t...
(A) At 96 h post-transfection with siRPAP3, cell viability was determined and calculated as a percen...
<p>(A) Viability assay (WST-1) on 911 and U118MG cells. Cells were mock infected (black bar) or infe...
At 48 h post-transfection with either siRPAP3 or siNC, A549/SeV-C and A549/Ctrl cells were infected ...