Confocal microscopy staining of three and six day old biofilms.

<p>Biofilms attached to plastic coverslips were stained using the FilmTracer™ LIVE/DEAD® biofilm viability stain. The images are Z-stack projections indicating the thickness of the biofilms for strain R20291 and 630Δerm for both three day and six day old biofilms. The depth of the Z-stack is indicated below the images in µm. These are representative Z-stacks from three independent replicates.