Biofilm formation analysis with confocal laser scanner microscopy.

Images represent Three-dimensional reconstruction of 5-day old static biofilms; the blue fluorescence signal represents exopolysaccharides staining. Gray images are Intensity surface plots corresponding to the emitted fluorescence by CWC. The biofilm formed was directly observed using Nikon confocal microcopy C2+ with a 20X objective (CFI Plan ApVC 20X numerical aperture of 1.2). Exciting laser intensity, background level contrast, and electronic zoom were maintained at the same level.