Laser Doppler monitoring during reperfusion.

<p><b>A</b>: Representative brain from a pup killed at 48 hours after ischemia showing a pale delineated lesion (white dotted line) and 3 regions of interest (ROI), and representative cresyl violet-stained section showing a cortical infarct and 2 ROI for rCBF measurements in the penumbra with the laser probe. <b>B</b>: Changes in rCBF (mean of 3 ROI ± S.D) in 6 animals subjected to ischemia-reperfusion. Upon MCA occlusion, the rCBF dropped to 55±8% of baseline, and additional bilateral CCA occlusion further decreased rCBF to 18±5%. After CCA release, a gradual reperfusion was observed from 30±11% at 1 min to 44±9% 20 min after. *p<0.05, ** p<0.01 <i>vs</i> basal.