Assessment of stroke volume by post-mortem 2,3,5-TTC staining in brain slices from mice 24 hours after reperfusion following a 1 hour transient MCA occlusion.

<p>(A) representative 2,3,5-TTC stained brain slices. The white scale bar corresponds to 1 cm (upper panel). Mean infarct volumes (lower panel). (B) Mean edema volumes. (C) mean bleeding volumes, as assessed by measuring cyano-methemoglobin content of tissue extracts. Means ± SEM from n = 9 animals per group are given, * denotes significant differences of p<0.05 compared to Fc. At the beginning of reperfusion, mice received either Revacept (1 mg/kg) or an equimolar amount of Fc only (0.33 mg/kg), or rtPA (10 mg/kg), or a combination of Revacept and rtPA, intravenously.