Expression of AT3-291Δ and AT3-Q55 and cytotoxicity.

<p>A) Growth curves of <i>E. coli</i> strains expressing BLG (yellow dots), AT3-291Δ (blue dots), AT3-Q24 (black stars) and AT3-Q55 (red squares). Cultures were induced with 500 µM IPTG at OD₆₀₀ = 0.1 and growth profiles monitored at 37°C. Error bars represent standard deviations and are derived from at least five independent experiments. B) Changes in optical density per hour (ΔOD) derived from growth profiles of AT3-291Δ (291Δ), AT3-Q24 (Q24) and AT3-Q55 (Q55). C, D) Effect of removal of the inducer on growth rates of AT3-291Δ (C) and AT3-Q55 (D). After 2 h from the induction, cells were resuspended in an inducer-deprived medium (stop, empty bars). As a control, they were further cultured in the presence of the inducer (ind, full bars). ΔODs were normalized with respect to the growth profile of AT3-Q24.