Validation of PSC peaks in control and mitotic cells.

<p>A) Plot of the percentage of mitotic PSC peaks per decile of corresponding ranked control PSC sites indicates mitotic peaks tend to be, but are not exclusively, the highest ranked peaks. Control PSC peaks are ranked by p-value, number of sequence reads per peak, peak height and fold over background. B) Average profile plot at control peak regions (left panel) or mitotic peak regions (right panel) for PSC in control cells (gray line), PSC in mitotic cells (red line) and 5% of the PSC profile in control cells in peak regions (gray dashed line) shows that the average plot for PSC in mitotic cells is greater than that for 5% of the PSC profile in control cells, indicating the mitotic peaks are likely not due to contamination of the mitotic population with up to 5% of interphase cells. C) ChIP-qPCR for PSC in control cells at eight binding sites identified by ChIP-SEQ and a negative site confirms PSC binding at the ChIP-SEQ identified sites but not the negative site in control cells. D) ChIP-qPCR for PSC in mitotic cells at eight binding sites identified by ChIP-SEQ and a negative site confirms PSC binding at the ChIP-SEQ identified sites but not the negative site in mitotic cells. ∧ <i>P</i><0.05 (student's <i>t</i>-test). E) ChIP-qPCR for H3 in control and mitotic sites at eight binding sites identified by PSC ChIP-SEQ.