p300 is required for retention of enhancer and promoter bound PIC components in mitotic cells.

<p>A schematic of the locations of enhancers (−28.9 kb & −19.3 kb), upstream (−6.8 kb) and promoter (−0.2 kb) at the <i>FOS</i> locus relative to TSS as indicated. Position dependent profile at the <i>FOS</i> locus at indicated position (relative to TSS) for indicated antibodies (+) and no antibody control (−) as indicated determined by quantitative ChIP analysis. (A) Jurkat cells were treated with nocodazole (400 ng/ml) for 24 h to obtain metaphase (M-phase) population. Error bars represent standard error of mean from 3 biological replicates each determined in duplicate. (B) p300 WT and p300 KO in HCT 116 cells were treated with nocodazole (100 ng/ml) for 16 h to obtain metaphase (M-phase) population. Error bars represent standard error of mean from 2 biological replicates each determined in duplicate.