Effects of EGF on miRNA levels of miR155 and miR200c in Caski Cells.

<p>A. The relative expression levels of miR155 and miR200c were detected by real-time PCR. miR155 was upregulated with 3 days of EGF treatment (50 ng/ml), and no change was found in miR200c expression level under EGF stimulation. The relative miR155 expression ratio in EGF treatment/Control is 12.21. Experiments were repeated 3 times. T-test was used for statistical analysis; ** P<0.01. B. The upregulated miR155 expression level was verified by reverse-transcription PCR. U6 was used as an internal control, Lane 1 and 2 are EGF treatment, lane 3 is negative control, 4 lane is marker, lane 5 and 6 are control (without EGF).