Expression and localization of EGFP-RPS9 fusion proteins.

<p>A.) U2OS osteosarcoma cells were transfected with EGFP-RPS9 deletion mutants as indicated in the figure and fixed 24 hours after transfection. Corresponding DAPI images are also shown. Bar 10 µM. B.) Western blotting demonstrates expression levels of the different EGFP-RPS9 fusion proteins. EGFP-RPS9 was detected using anti-GFP antibody and loading was verified with a β-actin antibody. C.) Schematic representation of the different fusion proteins tested and their localization pattern within cells. The patterns were defined as being predominantly nucleolar, nucleolar and cytoplasmic, even nuclear, or even throughout the entire cell. Shown is the percentage of cells with a particular staining pattern, representing the analysis of 200 transfected cells for each transfected plasmid.