Erythroid/Gata1 precursors are affected by <i>csrnp1a</i> knockdown.

<p>(A, C, E) Control and (B, D, F) morphant embryos. At 8 somite stage the expression of the erythroid marker <i>klf4</i> was not affected in morphant embryos (A, B) (100%, n = 48). However a clear decrease in <i>gata1</i> expression was detected in <i>csrnp1a</i> deficient embryos (compare C with D) (54,8%; n = 23/42) without major changes in the number of <i>myoD</i> stripes (7,1%; n = 3/42, more than 2 stripes), which served as readout of developmental progression. Control embryos showed normal <i>gata1</i> expression in 100% of the cases (n = 0/27) and 3,7% have altered <i>myoD</i> expression (n = 1/27). In agreement with <i>gata1</i> inhibition, at 32 hpf terminal differentiation marker, <i>hbae1</i>, was reduced in morphant embryos (E, F) (32,6%; n = 14/43).