Correlation of GATA1 occupancy with absolute RP expression levels in distinct stages of mouse and human terminal erythropoiesis.

<p>(A) The absolute mRNA expression levels of RP genes in morphologically distinct stages of mouse terminal erythroid differentiation are shown. GATA1 occupancy in early (Ter119-) and late (Ter119+) erythroid differentiation stages and PU.1 occupancy in mEsEPs cells is also included. (B) The quantification of absolute mRNA levels between GATA1 occupied (red) and non-GATA1 occupied (grey) RP genes consistently shows a significant association of GATA1 binding with higher expressed RP genes (red), despite the overall decline in RP gene expression with erythroid differentiation (grey). (C) The absolute mRNA expression levels of RPs in morphologically distinct stages of human terminal erythroid differentiation and GATA1 occupancy in fetal and adult derived human erythroid cells is shown. (D) The quantification of absolute mRNA levels between GATA1 occupied (red) and non occupied (grey) RPs again shows a consistently significant association of GATA1 binding with higher expressed RP genes (red), despite the overall decline in RP gene expression with erythroid differentiation (grey).