<p>Wild type, ΔBF1576, and ΔBF2185 strains of <i>B. fragilis</i> were cultured in DMM supplemented with varying concentrations of Pi as indicated. Total RNA was extracted from mid-logarithmic phase cultures, and <i>pstC</i> expression levels were compared by qPCR. The transcriptional level of <i>pstC</i> was normalized with that of <i>rpoD</i> and shown relative to that of a wild type strain in DMM supplemented with 6.6 mM KH<sub>2</sub>PO<sub>4</sub> (Pi-rich media). Black column: wild type; gray column: ΔBF1576; white column: ΔBF2185. **Significantly different from the wild type strain (<i>p</i><0.01).</p
<p>(A, B) Fold change expression ratio of 9 genes encoding TFs in mutant FGSC 9568 <i>vs</i> FGSC 24...
<p>The transcriptional levels of representative genes in wild type (closed column) and <i>phoB</i> d...
<p>The expression of NtrX in different media was determined by qRT-PCR and Western blot. (A) qRT-PCR...
<p>(A) Growth curve in DMM supplemented with varying concentrations of KH<sub>2</sub>PO<sub>4</sub>....
<p>(A) Construction of ΔBF1576 and ΔBF2185 strains. Internal deletion of each gene was confirmed by ...
<p>(A) Western blot analyses of PhoB expression. Wild type strains BW25113 (WT) and RU1622 (WT-<i>yf...
<p>Low Pi and PhoB increased transcription of <i>stx2</i> and its toxin production and of BP933W gen...
<p>Quantitative real-time PCR was employed to measure gene expression levels in the wild-type strain...
<p>(A) Classification of genes whose expression levels were altered more than 4-fold under Pi limita...
<p>The <i>FPS</i> (A), <i>crtE</i> (B), <i>crtS</i> (C) and <i>crtR</i> (D) genes expression in tran...
<p>VSMCs were cultured for 10 days in DMEM supplemented with 1% FBS, in the presence or absence of 3...
<p>The fold changes in expression in the <i>B. pseudomallei</i> K96243 wild-type (▪) and <i>bprD</i>...
A) RT-qPCR analysis of expression of 3 genes from the PhoP regulon. Bars represent fold changes in t...
<p>Growth (continuous lines, dry biomass per plate) and concentration of Pi in the growth medium (do...
<p>Five different media (Power, Czapek, PDA, CYA and YES) were used. Error bars represent the standa...
<p>(A, B) Fold change expression ratio of 9 genes encoding TFs in mutant FGSC 9568 <i>vs</i> FGSC 24...
<p>The transcriptional levels of representative genes in wild type (closed column) and <i>phoB</i> d...
<p>The expression of NtrX in different media was determined by qRT-PCR and Western blot. (A) qRT-PCR...
<p>(A) Growth curve in DMM supplemented with varying concentrations of KH<sub>2</sub>PO<sub>4</sub>....
<p>(A) Construction of ΔBF1576 and ΔBF2185 strains. Internal deletion of each gene was confirmed by ...
<p>(A) Western blot analyses of PhoB expression. Wild type strains BW25113 (WT) and RU1622 (WT-<i>yf...
<p>Low Pi and PhoB increased transcription of <i>stx2</i> and its toxin production and of BP933W gen...
<p>Quantitative real-time PCR was employed to measure gene expression levels in the wild-type strain...
<p>(A) Classification of genes whose expression levels were altered more than 4-fold under Pi limita...
<p>The <i>FPS</i> (A), <i>crtE</i> (B), <i>crtS</i> (C) and <i>crtR</i> (D) genes expression in tran...
<p>VSMCs were cultured for 10 days in DMEM supplemented with 1% FBS, in the presence or absence of 3...
<p>The fold changes in expression in the <i>B. pseudomallei</i> K96243 wild-type (▪) and <i>bprD</i>...
A) RT-qPCR analysis of expression of 3 genes from the PhoP regulon. Bars represent fold changes in t...
<p>Growth (continuous lines, dry biomass per plate) and concentration of Pi in the growth medium (do...
<p>Five different media (Power, Czapek, PDA, CYA and YES) were used. Error bars represent the standa...
<p>(A, B) Fold change expression ratio of 9 genes encoding TFs in mutant FGSC 9568 <i>vs</i> FGSC 24...
<p>The transcriptional levels of representative genes in wild type (closed column) and <i>phoB</i> d...
<p>The expression of NtrX in different media was determined by qRT-PCR and Western blot. (A) qRT-PCR...