Effect of BF1576 deletion on the growth in Pi-limiting media.

<p>(A) Construction of ΔBF1576 and ΔBF2185 strains. Internal deletion of each gene was confirmed by PCR using a primer pair encompassing each deletion site. W, wild type <i>B. fragilis</i>; M, mutant form of indicated gene; S, 100-bp ladder size markers. (B) Growth of wild type (WT), ΔBF1576 and ΔBF2185 strains under Pi-rich and Pi-limiting conditions. WT (diamonds), ΔBF1576 (squares), and ΔBF2185 (triangles) strains of <i>B. fragilis</i> were grown anaerobically in DMM supplemented with 6.6 mM (closed symbols) or 0.0066 mM (open symbols) of KH₂PO₄. Growth was measured at OD₆₀₀. Data presented are the means ± standard deviations of triplicate cultures. **Significantly different from wild type (<i>p</i><0.01), *Significantly different from wild type (<i>p</i><0.05).