Protocol 2- Th1, Th2, and Th17 cytokines and chemokines in the BALF after chronic HDM exposure.

<p><i>A</i>, INF-γ was suppressed after 6 weeks of HDM exposure and in the rapamycin (Rapa) treated HDM group (<i>n = </i>3–5 mice/group). *<i>p</i><0.05 versus saline; ^#<i>p</i><0.05 versus Dex. <i>B</i>, No significant differences in IL-17A levels were observed between animal groups, although there were trends for increased IL-17 in the HDM exposed group and lower leves in mice exposed to HDM and treated with Rapa or dexamethasone (Dex) during weeks 4–6 (<i>n = </i>3–5 mice/group). <i>C</i>, IL-13 levels were not significantly altered with HDM exposure, by Rapa or by Dex treatment in this model (<i>n = </i>3–5 mice/group). <i>D</i>, IL-4 levels were increased after 3 weeks of HDM compared to saline controls. After 6 weeks of HDM, IL-4 levels were higher in the Rapa treated group. IL-4 levels were similar between HDM and Dex treated mice (<i>n = </i>3–5 mice/group). *<i>p</i><0.05 versus saline; ∧<i>p</i><0.05 versus HDM; ^#<i>p</i><0.05 versus Dex. <i>E</i>, No statistically significant differences in IL-5 levels were observed between any group of mice (<i>n = </i>3–5 mice/group). <i>F</i>, Eotaxin 1 levels were increased after 3 weeks of HDM exposure and were higher in the Rapa treated group after 6 weeks of chronic HDM compared to saline controls (<i>n = </i>3–5 mice/group). *<i>p</i><0.05 versus saline.