Expression of P-Rex1 and PDGFRβ are required for invasive migration of WM852 melanoma cells.

<p>(A) Immunoblot showing depletion of P-Rex1 or PDGFRβ following transfection of cells with non-targeting control or siRNA oligonucleotides directed against P-Rex1 or PDGFRβ. (B,C) Invasive migration of WM852 cells into Matrigel following transfection with siRNA oligonucleotides targeting P-Rex1 or PDGFβ was determined as for Fig. 3A. In (C), panels have been appended to demonstrate the adherence of the WM852 cells to the lower surface (LS) of the transwell.(±SEM, ***<i>P<</i>0.0005 Mann-Whitney rank sum test, 3 independent experiments).