Colocalization, Western Blot and co-IP of NS80 and NS38 in transfected cells. A

<p>. IF microscopy of single transfection with pCI-neo-NS38 (up row) and cotransfection with pCI-neo-NS80 and pCI-neo-NS38 at 6 h, 12 h, 18 h p.t. The subcellular localization of NS80 was visualized by immunostaining with rabbit anti-NS80 serum followed by FITC-conjugated goat anti-rabbit IgG (green), NS38 was detected by immunostaining with mouse anti-NS38 polyclonal antibody followed by Texas Red-conjugated goat anti-mouse IgG (red). Nuclei were counterstained with DAPI (blue). <b>B</b>. IF microscopy of Vero cells cotransfected with pEGFP-C1-NS80 and pCI-neo-NS38 (top row) or pEGFP-C1-NS38 and pCI-neo-NS80 (bottom row). Cells were immunostained with corresponding antibodies. <b>C</b>. IB analysis of NS80 or NS38 in Vero cells. Cells were singly transfected or cotransfected with plasmids pCI-neo-NS80 and pCI-neo-NS38. Transfected cells were collected at 20 h p.t., lysed and analyzed by immunoblotting. <b>D</b>. Co-IP assay of NS80 and NS38 in transfected Vero cells. The transfected cells were harvested at 20 h p.t., lysed and immunoprecipitated with NS38-specific rabbit antiserum (upper panel) or NS80-specific mouse antiserum (lower panel), immunoprecipitated proteins were analyzed using IB analysis as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055334#pone-0055334-g004" target="_blank">Fig. 4C</a>.