Immunofluorescence analysis of the distributions of N-terminal truncated NS80 and VP1, VP2, VP3, or VP4 when coexpressed.

<p>For all the pictures, green fluorescence indicated N-terminal truncated NS80, and red fluorescence indicated VP1, VP2, VP3, or VP4. The nucleus was stained by Hoechst 33342 and presented as blue. Colocalized proteins were presented as yellow in merged figures. Bar = 20 μm. A. Immunofluorescence analysis of coexpression of VP1 and N-terminal truncated NS80. NS80-Δ55 and NS80-Δ216 colocalized with VP1 in the cytoplasm, but NS80-Δ512 and NS80-Δ549 colocalized with VP1-HA in the nucleus. B. Immunofluorescence analysis of the coexpressed VP2 and N-terminal truncated NS80. NS80-Δ55 and NS80-Δ216 colocalized with VP2 in the cytoplasm but NS80-Δ512 (partially) and NS80-Δ549 colocalized with VP2 in the nucleus. C. Immunofluorescence analysis of coexpressed VP3 and N-terminal truncated NS80. NS80-Δ55, not NS80-Δ85, colocalized with VP3 in the cytoplasm. D. Immunofluorescence analysis of coexpressed VP4 and N-terminal truncated NS80. NS80-Δ55 and NS80-Δ216 colocalized with VP4 in the cytoplasm, but NS80-Δ512 and NS80-Δ549 colocalized with VP4 in the nucleus.