Photo-conjugation of Z domain probes to mouse IgG₁.

<p>(<b>A</b>): Analysis by SDS-PAGE of the mouse IgG₁ mAb photocoupling efficiency of nine different Z_WT- or Z_F5I-based probes, differing in the position of the maleimide benzophenone (MBP) group. As indicated, variants containing the MBP group at positions 2, 3, 4, 9, 11, 15, 24 or 32 were investigated. (<b>B</b>): Analysis by SDS-PAGE of the photocoupling efficiency of the Z_WT-Q32C-MBP and Z_F5I-Q32C-MBP probes, both containing the MBP group at position 32, to 19 different mouse IgG₁ mAbs (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056597#s4" target="_blank">Material and Methods</a> for a list). The designations HC+P, HC, LC and P, refer to heavy chain+probe, heavy chain, light chain and probe, respectively. The M lanes refer to marker protein with molecular weights in kDa as indicated.