<p>A. Wild-type and <i>gea1</i>+/− cells were stained with calcofluor white to visualize septa and imaged by fluorescence microscopy. Scale bar, 10 µM. B. Quantification of A. Cells were scored as having abnormal septa if multiple septa were present, or if the septum was mislocalized, abnormally thick, or forked. Error bars represent mean ± SD from 3 independent experiments. C. Wild-type and <i>gea1</i>+/− cells were subjected to transmission electron microscopy to visualize septum defects. Representative images of multi-septated cells and septa with morphological abnormalities are shown. Scale bar, 1 µm.</p
<p>A–D: representative fields of wildtype and the three mutant strains stained with Calcofluor and D...
<p><b>A</b>. The wild type and mutant cells were stained using the membrane dye FM4-64, and the cell...
<p>(<b>A, B</b>) Single-channel photomicrographs of double-fluorescence immunohistochemistry, showin...
<p>A. Wild-type and <i>gea1+/−</i> cells were stained with 5 µM BODIPY FL C<sub>5</sub>-ceramide to ...
<p>EM micrographs (A-C) and quantification (D) showing septum and cell-wall defects in <i>81nmt1-sbg...
<p>Based on the TEM data, the average length of the <i>msp1</i> mutant cell chains was estimated to ...
<p>(<b>A</b>) Distribution of septa was investigated under the microscope after 18 h and 32 h past i...
<p>Cell wall architecture and septum formation were visualized by TEM on JRS4 wild-type, JRS4Δ<i>yvq...
<p>(A) Calcofluor-labelled septa in the indicated <i>plo1</i><sup>+</sup> (top) and <i>plo1-ts19</i>...
<p><b>A</b> A kymograph showing the NLS-DsRed nuclear transport marker during mitosis and septation ...
<p>Organs imaged with Fluo-4 (a,d,g) were fixed and processed for SEM (b,c,e,f,h,i). (a, b, c) Cells...
<p><b>(A)</b> A Calcofluor White (CW) staining image of <i>pxl1</i>Δ cells with off-centered septa. ...
(A) Quantitation of the septal phenotypes in various septum positioning and polarity mutants. Cells ...
<p>Mid-intestinal images of 104- and 120-hpf wild type (A, D), agr2–5 mmMO1 and 5 mmMO2-coinjected (...
<p><b>A.</b> Logarithmically growing cells were stained with vital dye FM4-64 for 60 min and viewed ...
<p>A–D: representative fields of wildtype and the three mutant strains stained with Calcofluor and D...
<p><b>A</b>. The wild type and mutant cells were stained using the membrane dye FM4-64, and the cell...
<p>(<b>A, B</b>) Single-channel photomicrographs of double-fluorescence immunohistochemistry, showin...
<p>A. Wild-type and <i>gea1+/−</i> cells were stained with 5 µM BODIPY FL C<sub>5</sub>-ceramide to ...
<p>EM micrographs (A-C) and quantification (D) showing septum and cell-wall defects in <i>81nmt1-sbg...
<p>Based on the TEM data, the average length of the <i>msp1</i> mutant cell chains was estimated to ...
<p>(<b>A</b>) Distribution of septa was investigated under the microscope after 18 h and 32 h past i...
<p>Cell wall architecture and septum formation were visualized by TEM on JRS4 wild-type, JRS4Δ<i>yvq...
<p>(A) Calcofluor-labelled septa in the indicated <i>plo1</i><sup>+</sup> (top) and <i>plo1-ts19</i>...
<p><b>A</b> A kymograph showing the NLS-DsRed nuclear transport marker during mitosis and septation ...
<p>Organs imaged with Fluo-4 (a,d,g) were fixed and processed for SEM (b,c,e,f,h,i). (a, b, c) Cells...
<p><b>(A)</b> A Calcofluor White (CW) staining image of <i>pxl1</i>Δ cells with off-centered septa. ...
(A) Quantitation of the septal phenotypes in various septum positioning and polarity mutants. Cells ...
<p>Mid-intestinal images of 104- and 120-hpf wild type (A, D), agr2–5 mmMO1 and 5 mmMO2-coinjected (...
<p><b>A.</b> Logarithmically growing cells were stained with vital dye FM4-64 for 60 min and viewed ...
<p>A–D: representative fields of wildtype and the three mutant strains stained with Calcofluor and D...
<p><b>A</b>. The wild type and mutant cells were stained using the membrane dye FM4-64, and the cell...
<p>(<b>A, B</b>) Single-channel photomicrographs of double-fluorescence immunohistochemistry, showin...