Organellar morphology in <i>gea1+/−</i> cells.

<p>A. Wild-type and <i>gea1+/−</i> cells were stained with 5 µM BODIPY FL C₅-ceramide to label the Golgi or with 32 µM FM4-64 to label the vacuole. Staining was visualized by fluorescence microscopy. Scale bar, 10 µM. B. The pixel area associated with the largest vacuole was measured using Image J for individual wild-type (n = 262) and <i>gea1+/−</i> (n = 225) cells. The percentage of cells with vacuolar sizes of the indicated ranges was plotted using SigmaPlot. C. Wild-type (A) and <i>gea1</i>+/− cells (B) were subjected to transmission electron microscopy to visualize membranous structures. Representative images show flat ribbon-like structures consistent with Golgi membranes (insets) that appear similar in wild-type and <i>gea1+/−</i> cells. N, nucleus. Scale bar, 500 nm. D. Cells were labeled with FM4-64, followed by incubation in H₂O for 90 min to induce vacuolar fusion. Scale bar, 10 µM.