ANDV infects and replicates in PMEFs.

<p>(A) PMEFs were infected with ANDV (MOI = 0.4), fixed 1, 2, and 3 dpi, stained with an anti-N antibody, and imaged by fluorescence microscopy at 10×. The inset on 3 dpi is a 40× magnification. (B and C) Quantitative RT-PCR detection of ANDV S-segment viral RNA in PMEFs using TaqMan probes. (B) Cellular and (C) supernatant RNA was isolated at 1 h, 1, 2, and 3 dpi. Standards were serial dilutions of known copy numbers of ANDV S-segment <i>in vitro</i>-transcribed RNA, and samples were standardized by normalizing total input RNA per sample to copies/ng cell lysate RNA or copies/µL of supernatant RNA. Experiments for qRT-PCR were performed in triplicate and data represents the mean and SEM.