Impact of primer tailing on the panFMDV-5UTR RT-qPCR assay using normal or hot-start dNTPs.

<p>Viral genomic RNA of FMDV isolate SAT3/MAL/3/76 was tested with the panFMDV-5UTR RT-qPCR assay using different primer combinations in the presence of either normal dNTPs (panel A) or hot-start dNTPs (panel B). Non-linear regression models were fitted to the raw fluorescence data of each replicate and the resulting models were amalgamated into a single replicate model using the replist function from the qpcR package [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164463#pone.0164463.ref038" target="_blank">38</a>] (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164463#pone.0164463.s004" target="_blank">S4 File</a>). The figures show the replicate model of each primer combination with error bars representing 1 standard deviation (nt: non-tailed, t: tailed).