Association of STAT3-GFP with cytoplasmic sequestering endosomes in IL-6-treated Hep3B cells distinct from COX IV-positive mitochondria.

<p>Panel A. Hep3B cells in a 6-well plate were transfected with a combination of STAT3-GFP and STAT3-DsRed expression vectors and one day later imaged live without or with exposure to IL-6 for 30 min. (For convenience of subsequent imaging, at 30 min the cultures were fixed using 4% paraformaldehyde, pH 7.4, for 1 hr, washed and stored in PBS; live cell and fixed cell data were indistinguishable). Scale bar = 10 µm. Panel B. Clone 78 Hep3B cells in a 6-well plate were transfected with STAT3-GFP expression vector and one day later exposed to IL-6 for 30 min and then fixed. The cultures were then immunostained for COX IV using the rabbit pAb. Scale bar = 10 µm.