Targeting of STAT6-GFP but not of N1-GFP to mitochondria in Hep3B cells assayed in live-cells using TMRE as the mitochondrial vital stain.

<p>Hep3B cells in 35–mm plates were transfected with expression plasmids for N1-GFP (Panel A) or STAT6-GFP (Panel B) and 2 days later the cultures were exposed to TMRE at 5 nM for 15 min. After washing with PBS the live cells were imaged using a 40x water-immersion objective by two-color fluorescence in green (N1-GFP or STAT6-GFP) and red (TMRE). Insets are shown at high magnification within each panel. Scale bars = 10 µm except in Panel B, insets = 5 µm.