qPCR validation of gene expression for selected genes relevant to intrinsic apoptosis.

Alejandra Idan Valencia-Cruz (286040)
Laura I. Uribe-Figueroa (286041)
Rodrigo Galindo-Murillo (286042)
Karol Baca-López (286043)
Anllely G. Gutiérrez (286044)
Adriana Vázquez-Aguirre (286046)
Lena Ruiz-Azuara (286048)
Enrique Hernández-Lemus (286050)
Carmen Mejía (286052)

Publication date

February 2013

DOI

10.1371/journal.pone.0054664.g013

Abstract

<p>Panel A presents results for total RNA integrity: Band 1 = Molecular weight marker, Band 2 = HeLa control (no-treatment), Band 3 = HeLa treated with Cas IIgly (40 -M, 6 h). Panel B display endpoint qPCR: Bands 1: Molecular weight marker, 2: Bax (HeLa control); 3: Bcl-2 (HeLa control); 4: caspase-8 (HeLa control); 5: cyt C (HeLa control); 6: caspase-3 (HeLa control); 7: caspase-3 (HeLa Cas IIgly); 8: cyt C (HeLa Cas IIgly); 9: Bax (HeLa Cas IIgly); 10: Bcl-2(HeLa Cas IIgly); 11: caspase-8 (HeLa Cas IIgly). Panel C quantitative PCR levels for cases (red) and controls (blue)(for numerical values please refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054664#pone-0054664-t004" target="_blank">Table 4</a>). qPCR...