Protein interaction between PdeIF(iso)4E and PPV-VPg.

<p>A. Y2H assay showing the interaction between plum eIF(iso)4E and PPV-VPg. BD and AD represent genes fused with GAL4 binding and activation domains, respectively. pGBKT7 and pGADT7-Rec represent empty vectors for bait and prey, respectively. SD/-Trp/-Leu and SD/-Trp/-Leu/-His/-Ade correspond to double dropout medium lacking Trp and Leu and quadruple dropout medium lacking Trp, Leu, His and Ade, respectively. Positive interactions result in yeast growth on the SD/-Trp/-Leu/-His/-Ade plus X-α-Gal plate and the development of the blue color. B. BiFC images confirming the positive interactions shown by Y2H. -YN, protein fused to N-terminal YFP; -YC, protein fused to C-terminal YFP. Scale bars = 38 µm.