Comparison of Pf4-Cre(neg);α2^flox/flox mice (f/f), heterozygous Pf4-Cre(neg);α2^+/flox mice (f/−) and α2-cKO mice (−/−).

<p>(<b>A</b>) Detection by western blot of α2 integrin in equivalent total protein samples from mononuclear cells (1, 2), platelets (3 – 6), lung (7, 8) and spleen (9, 10). Purified proteins were analyzed from f/f mice (1,3,7,9), f/− mice (5) and −/− mice (2,4,6,8,10). (<b>B,C</b>) Platelet parameters. Platelet count (<b>B</b>) and MPV (<b>C</b>) were measured in peripheral whole blood from f/f, f/− and −/− mice. The mean +/− SD are depicted (n = 6 in each case). The difference in MPV between f/f and −/− mice was statistically significant (p<0.01) (*).