TRPC3 in skeletal muscle arteries.

<p>A: skeletal muscle popliteal (left) and 1^st order (right) arteries immunolabeled for TRPC3 (top 3 panels) or no primary antibody control (bottom panel). The top image represents a composite of DAPI-stained nuclei in the endothelium, autofluorescence of the internal elastic lamina (green), and TRPC3 immunolabeling immunolabeling (red), and a compressed z-stack image endothelial cell surface (EC, top panel). The second image is a compressed z-stack image from the perspective of the smooth muscle face (SM), the third image is a cross-sectional slice, and the fourth is a compressed z-stack image of a no primary antibody control (NC).Red staining indicates endothelial expression of TRPC3. B: Magnified image of the cross-sectional view of the IEL. Note the sections highlighted by dotted square boxes indicating larger MEC and area of TRPC3 staining in 1^st order arterioles. Bar = 10 µm. Representative of images from tissue isolated from at least 3 animals.