Tenascin-C protein expression in vastus lateralis muscle.

<p><i>A</i>,<i>B)</i> Controls showing Tenascin-C specific straining (orange) of a large blood vessel (arteriole) in a muscle cross-section of an untrained participant after incubation with antibody MA3 (A) compared to incubation with a non-specific pre-immune serum (B). C) Tenascin-C (green, antibody B28.13) and CD31 (red) immuno-fluorescent staining, as well as co-localized tenascin-C and CD31 staining (yellow) in a section from a trained subject. A large blood vessel (most likely a venule) is identified based on CD31-staining and its thickened vessel wall. D,E) Tenascin-C staining (antibody MA3) in <i>vastus lateralis</i> muscle of a same participant before (D) and after (E) endurance training. Nuclei are stained in blue. Arrows and arrowheads point to tenascin-C staining in capillary structures and larger blood vessels (arterioles and venules). Bar = 50 μm.