<p>The uninduced and induced stages of expression of osmotin protein was resolved on 12% gel and stained with Coomassie brilliant blue. Lane M was loaded with Molecular weight marker. Lane U uninduced protein expression. Lane 1, 2, 3 shows expression after induction for 1 h, 2 h, 3 h respectively and lane P represents purified protein.</p
<p>(A) Lsa66, (B) Lp30 and (C) rLIC12238 expression from NaCl-induced <i>E. coli</i> Bl21-SI. M: mol...
<p>Lane 1, culture pellet (uninduced); Lane 2, culture pellet (induced with 0.3 mM IPTG at 16°C); La...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<p>(a) The proteins were resolved on 12% gel and stained with Coomassie brilliant blue. (b) SDS-PAGE...
Ed at 37°C for 3 h and induced by 0.4 mM IPTG (when indicated). . Molecular mass marker (LMW, Amersh...
Ed at 37°C for 3 h and induced by 0.4 mM IPTG (when indicated). . Molecular mass marker (LMW, Amersh...
<p>XeHypBA1 (A), XeHypBA2 (B), XeHypAA (C). M, protein molecular weight marker; lane 1, soluble prot...
<p>Purified proteins End (26.8 kDa) and XthA (32.1 kDa) were analyzed on a 12.5% SDS PAGE. Lane 1, p...
<p>(A) Quantification of protein expression and (B) Image of protein spots on gels. The left panel s...
<p>A) Lane M, protein molecular weight markers; lane 1, uninduced cell sample; line 2, induced cell ...
<p>A represented the SDS-PAGE of whole-cell protein. Lane 1, protein marker; lane 2, the cell withou...
<p>A and B. Coomasie blue stained gel. Lane M in A and B are protein molecular weight markers and th...
<p>Proteins that were present in the culture medium were subjected to SDS-PAGE and visualized by Col...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
<p>SDS-PAGE analysis of ONC-(P)GKY20 and ONC-DCless-H6-(P)GKY20 fusion proteins. Lane M1: marker (14...
<p>(A) Lsa66, (B) Lp30 and (C) rLIC12238 expression from NaCl-induced <i>E. coli</i> Bl21-SI. M: mol...
<p>Lane 1, culture pellet (uninduced); Lane 2, culture pellet (induced with 0.3 mM IPTG at 16°C); La...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<p>(a) The proteins were resolved on 12% gel and stained with Coomassie brilliant blue. (b) SDS-PAGE...
Ed at 37°C for 3 h and induced by 0.4 mM IPTG (when indicated). . Molecular mass marker (LMW, Amersh...
Ed at 37°C for 3 h and induced by 0.4 mM IPTG (when indicated). . Molecular mass marker (LMW, Amersh...
<p>XeHypBA1 (A), XeHypBA2 (B), XeHypAA (C). M, protein molecular weight marker; lane 1, soluble prot...
<p>Purified proteins End (26.8 kDa) and XthA (32.1 kDa) were analyzed on a 12.5% SDS PAGE. Lane 1, p...
<p>(A) Quantification of protein expression and (B) Image of protein spots on gels. The left panel s...
<p>A) Lane M, protein molecular weight markers; lane 1, uninduced cell sample; line 2, induced cell ...
<p>A represented the SDS-PAGE of whole-cell protein. Lane 1, protein marker; lane 2, the cell withou...
<p>A and B. Coomasie blue stained gel. Lane M in A and B are protein molecular weight markers and th...
<p>Proteins that were present in the culture medium were subjected to SDS-PAGE and visualized by Col...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
<p>SDS-PAGE analysis of ONC-(P)GKY20 and ONC-DCless-H6-(P)GKY20 fusion proteins. Lane M1: marker (14...
<p>(A) Lsa66, (B) Lp30 and (C) rLIC12238 expression from NaCl-induced <i>E. coli</i> Bl21-SI. M: mol...
<p>Lane 1, culture pellet (uninduced); Lane 2, culture pellet (induced with 0.3 mM IPTG at 16°C); La...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...