Expression in , purification, refolding and antifungal activity of an osmotin from -1

Ed at 37°C for 3 h and induced by 0.4 mM IPTG (when indicated). . Molecular mass marker (LMW, Amersham Pharmacia Biotech); . Total protein fraction from non-induced culture; . Total protein fraction from IPTG-induced culture; . Soluble protein fraction from IPTG-induced culture; . Insoluble protein fraction from IPTG-induced culture. . Western blot analysis of expressed His-tagged mature SnOLP probed with His-monoclonal antibody. . Soluble protein fraction from IPTG-induced culture; . Insoluble protein fraction from IPTG-induced culture. . SDS-PAGE analysis of insoluble His-tagged mature SnOLP which was urea solubilized and subsequently purified by immobilized-metal (Ni) affinity chromatography (IMAC). and . Eluates from IMAC. In . and . the gels were stained with Coomassie brilliant blue. His-tagged mature SnOLP protein is indicated by arrow heads.<p><b>Copyright information:</b></p><p>Taken from "Expression in , purification, refolding and antifungal activity of an osmotin from "</p><p>http://www.microbialcellfactories.com/content/7/1/7</p><p>Microbial Cell Factories 2008;7():7-7.</p><p>Published online 11 Mar 2008</p><p>PMCID:PMC2362109.</p><p>