<i>Beclin-1</i> is a target gene of miR-30a.

<p>(<b>A</b>) Sequence alignment between miR-30a and the 3′-UTR of <i>beclin</i>-<i>1</i> in several species. (<b>B</b>) The schematic diagram of the plasmid of pGL3-<i>Beclin-1</i> 3′-UTR-Wild Type and pGL3- <i>Beclin-1</i> 3′-UTR-Mutant. The wild-type and mutated sequences of the target site of miR-30a on the 3′-UTR of <i>Beclin-1</i> were shown. (<b>C</b>) Analysis of the relative luciferase activity in cardiomyocytes cotransfected with pGL3-<i>Beclin-1</i> 3′-UTR-Wild Type or pGL3-<i>Beclin-1</i> 3′-UTR-Mutant and miR-30a mimics, miR-30a inhibitors and negative control.*<i>P</i><0.05 compared with NC group. (<b>D</b>) Evaluation of the influence of miR-30a on mRNA level of <i>beclin-1</i> in cardiomyocytes. *<i>P</i><0.05 compared with NC group. (<b>E</b>) Evaluation of the influence of miR-30a on expression of beclin-1 and LC3II/LC3I proteins in cardiomyocytes. *<i>P</i><0.05 compared with NC group.