Chronic HDM exposure results in increased TSLP expression in mice.

<p>(A) The relative quantitative TSLP mRNA in the lung was detected using RT-PCR. (B) The TSLP protein concentration in the BALF was detected using an ELISA kit. The data shown represent the means±SEM (n = 5). mRNA value = 2^−ΔΔCt, ΔΔCt = (Ct_Target-Ct_Actin) of the treated mice-(Ct_Target-Ct_Actin) of the saline control mice. **p<0.01 compared with the HDM group. ^## p<0.01 compared with the IgG-treated control group. The results are derived from 4 experimental groups (5 mice/per group), and both the protein and mRNA levels were determined in duplicate.